D HUH7 cells. (TIF) S3 Fig. Uncropped Western blot of MTH1 in all tested cell lines. Basal expression of MTH1 in distinct neuroendocrine cell lines (BON1, H727, GOT1 and QGP1) and in HEPG2 and HUH7 cells. (TIF) S4 Fig. Uncropped Western blot of Actin and PCNA expression in combinational treatment. Expression of Actin and PCNA in neuroendocrine cell lines (BON1, H727 and QGP1) just after 96 h of incubation with TH588 (five M or 10 M) alone or in combination with 5FU (five M) or everolimus (10 nM). (TIF) S5 Fig. Uncropped Western blot of Caspase 3 and cleaved Caspase 3 expression in combinational therapy. Expression of Caspase three and cleaved Caspase 3 in neuroendocrine cell lines (BON1, H727 and QGP1) after 96 h of incubation with TH588 (five M or ten M) alone or in combination with 5FU (5 M) or everolimus (ten nM). (TIF) S6 Fig. Uncropped Western blot of PARP and cleaved PARP expression in combinational remedy. Expression of PARP and cleaved PARP in neuroendocrine cell lines (BON1, H727 and QGP1) right after 96 h of incubation with TH588 (five M or ten M) alone or in combination with 5FU (5 M) or everolimus (10 nM). (TIF) S7 Fig. Uncropped Western blot of Actin and PCNA expression in single substance treatment. Expression of Actin and PCNA in BON1 cells just after 24 h, 48 h and 72 h of incubationPLOS One particular | https://doi.org/10.1371/journal.pone.0178375 May possibly 25,12 /Effects of TH588 in NETswith TH588 (two,5 M, 5 M or 10 M). (TIF) S8 Fig. Uncropped Western blot of Caspase 3 and cleaved Caspase three expression in single substance treatment. Expression of Caspase three and cleaved Caspase three in BON1 cells just after 24 h, 48 h and 72 h of incubation with TH588 (two,five M, 5 M or 10 M). (TIF) S9 Fig. Uncropped Western blot of PARP and cleaved PARP expression in single substance therapy. Expression of PARP and cleaved PARP in BON1 cells immediately after 24 h, 48 h and 72 h of incubation with TH588 (2,five M, five M or 10 M).Pd 122 Chemscene (TIF) S10 Fig. Uncropped Western blot of Actin and PCNA expression in combinational treatment. Expression of Actin and PCNA in neuroendocrine cell lines (BON1, H727 and QGP1) after 96 h of incubation with TH588 (5 M or 10 M) alone or in combination with 5FU (5 M) or everolimus (ten nM). (TIF) S11 Fig. Uncropped Western blot of pEGFR, pIGFR, pAkt, pErk, pS6 and p4EBP1 expression in combinational treatment. Expression of pEGFR, pIGFR, pAkt, pErk, pS6 and p4EBP1 in neuroendocrine cell lines (BON1, H727 and QGP1) soon after 96 h of incubation with TH588 (five M or ten M) alone or in combination with 5FU (5 M) or everolimus (ten nM).3-Hydroxy-5-methoxybenzaldehyde custom synthesis (TIF) S12 Fig.PMID:23776646 Uncropped Western blot of pEGFR, pIGFR, pAkt, pErk, pS6 and p4EBP1 expression in combinational treatment. Expression of pEGFR, pIGFR, pAkt, pErk, pS6 and p4EBP1 in neuroendocrine cell lines (BON1, H727 and QGP1) immediately after 96 h of incubation with TH588 (five M or 10 M) alone or in mixture with 5FU (5 M) or everolimus (ten nM). (TIF) S13 Fig. Uncropped Western blot of Caspase 3 and cleaved Caspase three expression in combinational remedy. Expression of Caspase three and cleaved Caspase three in neuroendocrine cell lines (BON1, H727 and QGP1) after 96 h of incubation with TH588 (5 M or 10 M) alone or in combination with 5FU (five M) or everolimus (ten nM). (TIF) S14 Fig. Uncropped Western blot of Caspase 3 and cleaved Caspase 3 expression in combinational treatment. Expression of Caspase 3 and cleaved Caspase three in neuroendocrine cell lines (BON1, H727 and QGP1) after 96 h of incubation with TH588 (5 M or ten M) alone or in combination with 5FU (5 M) or everolimus (ten nM). (TIF.
